Structure, assembly and reading of centromeric chromatin
نویسندگان
چکیده
منابع مشابه
Centromeric Chromatin and Epigenetic Effects in Kinetochore Assembly
proteins, such as transcription factors, bind selectively to DNA via short but highly conserved binding sites, a second class of factors such as yeast centromere binding factor III (CBF3; Espelin et al., 1997) and the origin recognition complex (ORC) bind to large, poorly conGarth R. Wiens and Peter K. Sorger Department of Biology Massachusetts Institute of Technology Cambridge, Massachusetts 0...
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Semi-conservative segregation of nucleosomes to sister chromatids during DNA replication creates gaps that must be filled by new nucleosome assembly. We analyzed the cell-cycle timing of centromeric chromatin assembly in Drosophila, which contains the H3 variant CID (CENP-A in humans), as well as CENP-C and CAL1, which are required for CID localization. Pulse-chase experiments show that CID and...
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Much controversy exists regarding the structural organization of the yeast centromeric nucleosome and the role of the nonhistone protein, Scm3, in its assembly and architecture. Here we show that the substitution of H3 with its centromeric variant Cse4 results in octameric nucleosomes that organize DNA in a left-handed superhelix. We demonstrate by single-molecule approaches, micrococcal nuclea...
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n page 765, Ohzeki et al. present evidence for sequence specificity in assembling chromatin at mammalian centromeres. The sequence requirements imply that construction of centromeric chromatin is more than an epigenetic process. O Centromeric chromatin assembles at AT-rich repeats called ␣ satellite sequences, or alphoids. Type I alphoids contain boxes for the binding of CENP-B, one of several ...
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Centromeric nucleosomes contain a histone H3 variant called centromere protein A (CENP-A) that is required for kinetochore assembly and chromosome segregation. Two new studies, Jansen et al. (see p. 795 of this issue) and Maddox et al. (see p. 757 of this issue), address when CENP-A is deposited at centromeres during the cell division cycle and identify an evolutionally conserved protein requir...
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ژورنال
عنوان ژورنال: Current Opinion in Genetics & Development
سال: 2012
ISSN: 0959-437X
DOI: 10.1016/j.gde.2011.11.005